The IgY antibody against P . gingivalis gingipains inhibits alveolar bone resorption on rat experimental periodontitis

Content Provider	Semantic Scholar
Author	Shikaya, Souji
Copyright Year	2014
Abstract	Porphyromonas gingivalis has been implicated as an important pathogen in the development of adult periodontitis, and its colonization of subgingival sites is critical in the pathogenic process. The gingipains are cell surface Argand Lys-specific proteinases of the bacterium Porphyromonas gingivalis, which has been associated with periodontitis, a disease that results in the destruction of the teeth-supporting tissues. The aim of this study was to investigate the efficacy of egg yolk antibody against gingipains (IgY-GP) to the protection alveolar bone loss by P. gingivalis in rats. Rats were orally infected with P. gingivalis ATCC 33277, which was suspended in 5% carboxymethylcellulose. Each rat received 0.5 ml of the suspension by oral gavage. Then, the IgY-GP and the IgYControl in 5% carboxymethylcellulose were administered, with each rat receiving 0.5 ml by oral gavage. The upper jaws were used as dry specimens for measuring horizontal alveolar bone loss. The distance between the cementenamel junction (CEJ) and the alveolar bone crest (ABC) was measured at 14 palatal sites per rat. P. gingivalisinfected group showed an increased distance between CEJ and ABC compared to uninfected group, indicating an enhanced alveolar bone loss due to oral infection with P. gingivalis. However, administered the IgY-GP group showed a decreased distance between CEJ and ABC compared to P. gingivalis-infected group. The IgY-GP could be used for the development of passive immunization against P. gingivalis-associated periodontal disease. These findings suggested that the IgY-GP protects P. gingivalis–induced alveolar bone loss. Presented by Medical*Online 2014 年 6 月 IgY-GP のラット実験歯周炎に対する歯槽骨吸収抑制効果 17 生することが知られているが,なかでもジンジパイ ン(gingipains)は本菌の産生する主要なプロテアー ゼであり,ペプチド切断部位特異性の異なるArggingipain(Rgp) とLys-gingipain(Kgp) が 存 在 す る。これら2つの酵素は,宿主細胞に傷害を与え 歯周病に関連する種々の病態を生み出すと考えられて いる。ジンジパインは単量体として菌体細胞外に分泌 されるだけでなく,外膜上では血球凝集素やヘモグロ ビン結合タンパク質,LPS,リン脂質と結合した高分 子複合体としても存在することが知られている。 慢性歯周炎の原因菌がP. gingivalisであることは 多くの臨床的,実験的研究から明らかにされている。 我々の研究室においてもP. gingivalisをラット口腔 に接種することにより歯槽骨の吸収を惹起する実験 的歯周炎を用いて,P. gingivalisの病原因子の解析を 行なってきた。本研究においては,P. gingivalisの 産生する酵素であるジンジパインに対する鶏卵抗体 (Immunoglobulin Yolk;IgY)を用いて,ラット実験 歯周炎に対する骨吸収抑制効果について検討した。
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Alternate Webpage(s)	http://www.graduate.kdu.ac.jp/dessertation/pdf/h2509/%E4%B9%99%E7%AC%AC491%E5%8F%B7_P.%20gingivalis%20%E3%82%B8%E3%83%B3%E3%82%B8%E3%83%91%E3%82%A4%E3%83%B3IgY%E6%8A%97%E4%BD%93%E3%81%AE%E3%83%A9%E3%83%83%E3%83%88%E5%AE%9F%E9%A8%93%E6%AD%AF%E5%91%A8%E7%82%8E%E3%81%AB%E5%AF%BE%E3%81%99%E3%82%8B%E6%AD%AF%E6%A7%BD%E9%AA%A8%E5%90%B8%E5%8F%8E%E6%8A%91%E5%88%B6%E5%8A%B9%E6%9E%9C.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article