Identificaçao de bacterias laticas isoladas do ecossistema mamario bovino e caracterizacao de seu potencial inibidor contra patogenos associados à mastite

Content Provider	Semantic Scholar
Author	Assis, Bianca Seridan De
Copyright Year	2015
Abstract	Mastitis causes huge economic losses in the dairy sector both in Brazil and France. They also are the first cause of antibiotic consumption in the dairy farms. There is thus a need for new alternatives to antibiotics to control infectious mastitis. In this thesis work, we isolated 278 (165 in France and 113 in Brazil) bacterial strains from bovine milk and teat canal, and identified 10 non-redundant lactic acid bacteria (LAB) strains that were further tested as potential mammary probiotic candidates. LAB strains were tested for their surface properties and production of inhibitory compounds and then evaluated for their interactions with Staphylococcus aureus and Escherichia coli, two major mastitis pathogens, or with bovine mammary epithelial cells (bMEC), in vitro. Some LAB strains (Lactobacillus brevis e Lactobacillus plantarum) presented inhibitory capacity against S. aureus adhesion and internalisation and were shown to stimulate the production of pro- and anti-inflammatory cytokinin bMEC. Other interaction tests with bMEC showed that Lactococcus lactis V7 was able to significantly inhibit bMEC invasion by Escherichia coli and S. aureus. Although the inhibitory mechanism was not elucidated, Lactococcus lactis V7 showed promising capacities in terms of mammary probiotic potential with, notably, the ability to modulate the immune response of E. coli-infected bMEC by modifying the expression of inflammatory cytokine genes. In this work, several LAB strains were identified in the milk and teat canal microbiota. We showed that L. brevis, L. plantarum and Lactococcus lactis
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://tel.archives-ouvertes.fr/tel-01366410/document
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article