Loading...
Please wait, while we are loading the content...
Similar Documents
Molecular characterization and redox regulation of phosphoribulokinase from the cyanobacterium Synechococcus sp. PCC 7942.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kobayashi, Daisuke Tamoi, Masahiro Iwaki, Toshio Shigeoka, Shigeru Wadano, Akira |
| Copyright Year | 2003 |
| Abstract | We isolated and characterized a gene encoding phosphoribulokinase (PRK) from Synechococcus sp. PCC 7942. The isolated sequence consisted of a 999 bp open reading frame encoding 333 amino acid residues of PRK. The PRK contained a pair of cysteinyl residues corresponding to Cys16 and Cys55 of spinach PRK regulated by a ferredoxin-thioredoxin system. However, there were seventeen amino acid residues lacking between the two cysteinyl residues compared with those of the chloroplastic enzyme in higher plants. The recombinant PRK of Synechococcus sp. PCC 7942 accounted for about 6-13% of the total soluble protein in the Escherichia coli. The specific activity of the enzyme was 230 micro mol min(-1) (mg protein)(-1). The enzyme activity was completely inactivated by treatment with 5,5'-dithiobis (2-nitrobenzoic acid) (cysteinyl residue-specific oxidant) or was decreased by treatment with H(2)O(2), but was more tolerant to oxidation than that of chloroplast. The oxidized PRK was fully activated by treatment with excessive dithiothreitol. Furthermore, incubation with 3 mM ATP protected the oxidation of the enzyme by either 5,5'-dithiobis (2-nitrobenzoic acid) or H(2)O(2). These results suggest Synechococcus sp. PCC 7942 PRK can be regulated by reversible oxidation/reduction in vitro, but might be resistant to oxidative inactivation in vivo. |
| File Format | PDF HTM / HTML |
| DOI | 10.1093/pcp/pcg048 |
| PubMed reference number | 12668773 |
| Journal | Medline |
| Volume Number | 44 |
| Issue Number | 3 |
| Alternate Webpage(s) | https://watermark.silverchair.com/pcg048.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAjswggI3BgkqhkiG9w0BBwagggIoMIICJAIBADCCAh0GCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMtDhFDOKFcsszVWPbAgEQgIIB7gV9PxOqBQA2iBuwdFdw8dHmwGvxPyI6df7guKK-eWWlAT8tYeLNZYgidFyIvKJfTuadeHipuNx87YToZpliymgCQczp_XAHIrGYdrWZ2rytqWz8ibpOaI0AUogNgPMTpE_P8QQgtjdrDIobgVNxMvyegJrMoiEjq6Ome3GB5c6ecbyxgscBO7ObQN-R6_lG8WUdA5kGzuIZ6R1w8RspfJw1_kp4N2v8fOLym1pAKKIu7uRElOrDzIoee6UcOb7F5unpJQczSUaQuFM2t-OwM37YQdNvj52pVczW71NvyIOO_oUxLTdQhWPw0gCAXfc7I7JDUqnNXMTmqiOyjBVwm56lPIR1kKnHIOeXFKMukqZGH-63_kJ8VUEFb13YWn4_rHDwK1BmkxKtzD0eT23dez5q-bQgzNr_tu0vqnEocXek-uGATE-QjSJUcraUgzSSL2g3BsALQ-GZK2mCtCYIoGaraxFeXnbqYQSnEcSA8FpsnjxI3_Ip4qtk2naIDJGcmUlxFQJYAnZITdscFN9pxJBXUsKDL3sys9H3S31L6y0p3B0TNbtog7QR6vSGfeh3Op15xBLmUlcp_BcYOHhnJaMlJuh5so6HJRBVakLRtywgI0B2swMoG6KuXASv_J4gHMDM1dNlkxpsOh3fqi-8 |
| Alternate Webpage(s) | https://doi.org/10.1093/pcp%2Fpcg048 |
| Journal | Plant & cell physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
