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UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL INSTITUTO DE CIÊNCIAS BÁSICAS DA SÁUDE PROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIAS ANÁLISES COMPORTAMENTAL E MORFOLÓGICA DA ASSOCIAÇÃO DE EXPOSIÇÃO PRÉ-NATAL À LIPOPOLISSACARÍDEO E ASFIXIA INTRAUTERINA Dissertação de Mestrado
| Content Provider | Semantic Scholar |
|---|---|
| Author | Timmen, Maria Augusta Alegre, Raimundo Porto Raimundo, Maria Augusta Timmen Marcuzzo, Simone Aurizio, Federico D’ Raio, Rodrigo Raimundo, Timmen Raimundo, Rodrigo Daminello Marques, Gabriel García Souza, Samir Khal De Rossato, Marília |
| Copyright Year | 2016 |
| Abstract | The exposure of the developing brain to injuries such as maternal infections and perinatal asphyxia can induces permanent neurological deficits which may be potentially similar to those observed in cerebral palsy (CP). Since motor deficits observed in patients with CP are poorly reproduced in experimental models, the aim of this study was to examine if the combination of prenatal inflammation caused by lipopolysaccharide (LPS) and intrauterine asphyxia reproduces in rats functional and morphological changes similar to those found in patients with CP. Pups were divided in 4 groups: Control (CT), Asphyxia (A), LPS-injected (LPS) and LPS-injected plus asphyxia (LA). Intraperitoneal LPS (in LPS and LA groups) or sterile saline (in CT and A groups) injections was performed in pregnant rats between the 17th and 21st gestational days. At the 22nd gestational day, the asphyxia procedure was carried out in A and LA groups. All animals were weighed daily during the first 14 postnatal days (P14) and at P30. The developmental milestones were assessed between the P3 to P14. Animal’s motor skills were evaluated on the rotarod and grip test at P29. The repositioned object test, used to evaluate spatial memory, was also performed at P29. In the following day, the animals were euthanized and brain samples were collected. The caspase-3 and synaptophysin immunoreactivity in the dentate gyrus (DG) region of the hippocampus and myelin basic protein immunoreactivity in the striatum were analyzed. LPS and A groups showed less weight gain compared to control animals. LPS factor alone or in association with asphyxia delayed the acquisition of audio startle response and hindlimb proprioceptive placement, but accelerated the appearance of the forelimb grasp. No motor deficits were observed on rotarod and grip test. Deficits in spatial memory were also not verified. The DG morphological analysis showed that the LPS and LA group had a reduction in synaptophysin expression. Caspase-3 expression increased in DG of LA group compared to the control one. In striatum, no differences in mielin basic protein immunoreactivity was observed between the groups. Although the experimental model used increased cell death and decreased the synaptic activity in hippocampus, only subtle changes were observed in motor behavior abilities of the animals. Besides, the experimental approach used did not affect the myelin in the striatum, an |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://lume.ufrgs.br/bitstream/handle/10183/134855/000987354.pdf?sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
