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The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chang, C. Wang, S. H. Lin, Hsiang Ju |
| Copyright Year | 2001 |
| Abstract | Heterogeneous nuclear ribonucleoprotein K (hnRNP K), a component of hnRNP particles, is involved in several steps of gene expression regulation. Dengue (DEN) virus, a member of the Flaviviridae, is the primary cause of illnesses such as dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. In mature DEN virus particles, the core protein is a structural protein that forms a nucleocapsid complex with genomic RNA. Very little of its biologic functions is known. Here, using an in vitro binding assay and coimmunoprecipitation analysis, we report a protein-protein interaction between the DEN virus core protein and hnRNP K. The C-terminal hydrophilic region of the DEN virus core protein, spanning amino acid residues 73 to 100, is required for such interaction. Results of glutathione-S transferase binding assays indicated that the core protein-hnRNP K interaction might be abolished in the presence of hnRNP K cognate nucleic acids. Furthermore, in a cotransfection experiment, the repressive effect of hnRNP K on C/EBPbeta-mediated transcription activation could be reversed by full-length DEN virus core protein but not by a truncated form containing amino acids 1-72. Our results suggest that, on DEN virus infection, the multiple functions of cellular hnRNP K may be affected by the virus core protein. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ntur.lib.ntu.edu.tw/bitstream/246246/163267/1/08.pdf |
| PubMed reference number | 11747608v1 |
| Volume Number | 20 |
| Issue Number | 9 |
| Journal | DNA and cell biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Biological Factors Cell Nucleus Dengue Fever Dengue Shock Syndrome Dengue Virus Gene Expression Regulation Genetic Heterogeneity Glutathione Hemorrhagic Fevers, Viral Heterogeneous-Nuclear Ribonucleoprotein K Heterogeneous-Nuclear Ribonucleoproteins Illness (finding) Nucleic Acids Nucleocapsid RNA Staphylococcal Protein A Transcriptional Activation Transferase Vault Ribonucleoprotein Particles Virion Yellow Fever Vaccine hnRNP A1 protein protein interaction |
| Content Type | Text |
| Resource Type | Article |
