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Simple defined autoinduction medium for high-level recombinant protein production using T7-based Escherichia coli expression systems
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Zhao-Peng Kessler, Wolfgang Heuvel, Joop Van Den Rinas, Ursula |
| Copyright Year | 2011 |
| Abstract | Protein production under the control of lac operon regulatory elements using autoinduction is based on diauxic growth of Escherichia coli on lactose after consumption of more preferred carbon substrates. A novel simple and cost-effective defined autoinduction medium using a mixture of glucose, glycerol, and lactose as carbon substrate and NH4+ as sole nitrogen source without any supplementation of amino acids and vitamins was developed for T7-based E. coli expression systems. This medium was successfully employed in 96-well microtiter plates, test tubes, shake flasks, and 15-L bioreactor cultivations for production of different types of proteins achieving an average yield of 500 mg L−1 product. Cell-specific protein concentrations and solubility were similar as during conventional isopropyl β-d-1-thiogalactopyranoside induction using Luria-Bertani broth. However, the final yield of target proteins was about four times higher, as a higher final biomass was achieved using this novel defined autoinduction broth. |
| Starting Page | 1203 |
| Ending Page | 1213 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s00253-011-3407-z |
| Alternate Webpage(s) | https://hzi.openrepository.com/bitstream/handle/10033/214650/Li%20et%20al_final.pdf;jsessionid=CE8818431966088F847B6E4C0947BE9E?sequence=1 |
| PubMed reference number | 21698378 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00253-011-3407-z |
| Journal | Medline |
| Volume Number | 91 |
| Journal | Applied Microbiology and Biotechnology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
