NDLI: Nonsmokers Chromatid Exchanges in Human Lymphocytes from Smokers ) pyrene-diolepoxide : DNA Adduct Formation , and Sister a ) pyrene Metabolism , Benzo ( a Relationships among Benzo ( Updated

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Nonsmokers Chromatid Exchanges in Human Lymphocytes from Smokers ) pyrene-diolepoxide : DNA Adduct Formation , and Sister a ) pyrene Metabolism , Benzo ( a Relationships among Benzo ( Updated

Content Provider	Semantic Scholar
Author	Thompson, Claudia L. McCoy, Zadock Lambert, Jane M. Andries, Maria Lucier, George
Copyright Year	2006
Abstract	In the present study, benzo(a)pyrene (BP) metabolism, DNA adduct formation, ethoxyresorufin-0-deethylase activity, and sister chromatid exchange induction by BP were compared in human lymphocytes pre pared from u hole blood of smokers and nonsmokers following an in vitro incubation with BP. There was an approximate 7to 10-fold variation in all parameters measured. To determine the source of this variation, participants were resampled, the assays were repeated, and all the data were analyzed to assess (a) smoking-related effects, (b) differences in multiple samples from the same individual, and (c) intraindividual, ex perimental, and interindividual variation. No smoking-related effects were observed except for baseline sister chromatid exchange frequency. The variation observed for BP-related DNA adducts and ethoxyresorufin-0deethylase activity was primarily due to interindividual variation. For example, in vitro formation of DNA adducts did not change when samples were obtained at different times from the same individual and were not influenced significantly by culture conditions. No significant correlation existed between DNA adduct formation and BP metabolism [correlation coefficient (r) = 0.27\| for cither the total population or when segregated based on smoking status. Furthermore, no correlation was seen between DNA adducts and sister chromatid exchange induction by BP. Our studies have compared a number of commonly used lymphocyte markers and conclude that it is difficult to predict changes in one marker based on changes in another. However, in vitro formation of BP-derived DNA adducts is consistent over time for individuals.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cancerres.aacrjournals.org/content/49/23/6503.full.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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