NDLI: The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes.

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The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes.

Content Provider	Semantic Scholar
Author	Zheng, Yin Yuan Jiang, Yu
Copyright Year	2005
Abstract	Phosphotyrosyl phosphatase activator PTPA is a type 2A phosphatase regulatory protein that possesses an ability to stimulate the phosphotyrosyl phosphatase activity of PP2A in vitro. In yeast Saccharomyces cerevisiae, PTPA is encoded by two related genes, RRD1 and RRD2, whose products are 38 and 37% identical, respectively, to the mammalian PTPA. Inactivation of either gene renders yeast cells rapamycin resistant. In this study, we investigate the mechanism underling rapamycin resistance associated with inactivation of PTPA in yeast. We show that the yeast PTPA is an integral part of the Tap42-phosphatase complexes that act downstream of the Tor proteins, the target of rapamycin. We demonstrate a specific interaction of Rrd1 with the Tap42-Sit4 complex and that of Rrd2 with the Tap42-PP2Ac complex. A small portion of PTPA also is found to be associated with the AC dimeric core of PP2A, but the amount is significantly less than that associated with the Tap42-containing complexes. In addition, our results show that the association of PTPA with Tap42-phosphatase complexes is rapamycin sensitive, and importantly, that rapamycin treatment results in release of the PTPA-phosphatase dimer as a functional phosphatase unit.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.molbiolcell.org/content/16/4/2119.full.pdf
PubMed reference number	15689491v1
Volume Number	16
Issue Number	4
Journal	Molecular biology of the cell
Language	English
Access Restriction	Open
Subject Keyword	Less Than Mammals PPP2R4 gene Protein Phosphatase 2A Regulatory Subunit PR53 Protein Tyrosine Phosphatase Saccharomyces cerevisiae Sirolimus phosphoric monoester hydrolase activity regulatory proteins
Content Type	Text
Resource Type	Article

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