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Identifying G-quadruplex-binding ligands using DNA-functionalized gold nanoparticles.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qiao, Yunxia Deng, Jing Jin, Yan Chen, Guozhen Wang, Lin |
| Copyright Year | 2012 |
| Abstract | The G-rich overhang of human telomere tends to form a G-quadruplex structure, and G-quadruplex formation can effectively inhibit telomerase activity in most cancer cells. Therefore, it is important to identify the formation and properties of the G-quadruplex, with the particular aim of selecting G-quadruplex-binding ligands that could potentially lead to the development of anticancer therapeutic agents. With this goal in mind, we report a fluorescence resonance energy transfer (FRET) assay system for the identification of G-quadruplex ligands using DNA-functionalized gold nanoparticles (DNA-GNPs) as the fluorescence quencher and a carboxyfluorescein (FAM)-tagged human telomeric sequence (F-GDNA) as the recognition probe. A thiolated complementary strand of human telomeric DNA (cDNA), which first adheres to the surface of the GNPs and then hybridizes with F-GDNA, results in the fluorescence quenching of F-GDNA by the GNPs. However, fluorescence is restored when single-stranded F-GDNA folds into a G-quadruplex structure upon the binding of quadruplex ligands, leading to the release of F-GDNA from the surface of the GNPs. Combined data from fluorescence measurements and CD spectroscopy indicated that ligands selected by this FRET method could induce GDNA to form a G-quadruplex. Therefore, this FRET G-quadruplex assay is a simple and effective approach to identify quadruplex-binding ligands, and, as such, it promises to provide a solid foundation for the development of novel anticancer therapeutic agents. |
| Starting Page | 95 |
| Ending Page | 123 |
| Page Count | 29 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/an/c2/c2an16051j/c2an16051j.pdf |
| PubMed reference number | 22331167v1 |
| Alternate Webpage(s) | https://doi.org/10.1039/c2an16051j |
| DOI | 10.1039/c2an16051j |
| Journal | The Analyst |
| Volume Number | 137 |
| Issue Number | 7 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | DNA, Complementary Fluorescence Resonance Energy Transfer G-quadruplex DNA formation Genomic DNA Ligands Nucleic Acid Strand Telomerase cancer cell carboxyfluorescein |
| Content Type | Text |
| Resource Type | Article |
