NDLI: Activated polymorphonuclear cells increase sickle red blood cell retention in lung: role of phospholipids.

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Activated polymorphonuclear cells increase sickle red blood cell retention in lung: role of phospholipids.

Content Provider	Semantic Scholar
Author	Haynes, Johnson
Copyright Year	2002
Abstract	This study investigates the role of the activated polymorphonuclear cell (APMN) products on sickle red blood cell (SRBC) retention/adherence in the pulmonary circulation. Isolated rat lungs were perfused with (51)Cr-labeled normal RBCs (NRBC) or SRBCs (10% hematocrit) suspensions +/- PMNs. Specific activities of lung and perfusate were measured and retention (the number of SRBC/g lung) was calculated. SRBC retention was 3.5 times greater than NRBC retention. PMN activation was required to increase SRBC retention. Supernatants from APMN increased SRBC retention, which suggested soluble products such as oxidants, PAF, and/or leukotriene (LTB(4)) are involved. Heat inactivation of PMN NADPH oxidase had no effect on retention. Whereas neither platelet-activating factor (PAF) nor LTB(4) (secreted by APMN) increased SRBC retention, PAF+LTB(4) did. The PAF antagonist, WEB-2170, attenuated SRBC retention mediated by PAF+LTB(4) and APMNs. Similarly, zileuton (5-lipoxygenase inhibitor) attenuated APMN-mediated SRBC retention. We conclude the concomitant release of PAF and LTB(4) from APMN is involved in the initiation of microvascular occlusion by SRBCs in the perfused rat lung.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ajpheart.physiology.org/content/ajpheart/282/1/h122.full.pdf
PubMed reference number	11748055v1
Volume Number	282
Issue Number	1
Journal	American journal of physiology. Heart and circulatory physiology
Language	English
Access Restriction	Open
Subject Keyword	Arachidonate 5-Lipoxygenase Blood Cells Blood Platelets Cell secretion Erythrocytes Leukotrienes Oxidants Phospholipids Platelet Activating Factor Pulmonary Circulation Red Blood Cell Count measurement Structure of parenchyma of lung Suspensions Tracer WEB 2170 neutrophil zileuton
Content Type	Text
Resource Type	Article

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