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[Enhancing hRANKL production in Escherichia coli by optimizing culture conditions and lysing program].
| Content Provider | Semantic Scholar |
|---|---|
| Author | Che, Luyang Guo, Zhilan Guo, Qinghua Pan, Bing Yu Li, Jingzhe Liu, Changzhen Huang, Peng |
| Copyright Year | 2017 |
| Abstract | RANKL/RANK/OPG axis is important in bone metabolism regulation, and becomes a popular research area in bone diseases. RANKL is a critical part of RANKL/RANK/OPG axis, and widely required in bone metabolism research. However, the yield of recombinant soluble human RANKL (hRANKL) in Escherichia coli is much lower than mouse RANKL (mRANKL). In this study, by adjusting and stabilizing the pH value of LB medium at 7.5, lowering the inducing temperature to 16 ℃ and optimizing the lysis program, the yield of soluble hRANKL increased by approximately 5 to 12-fold over the non-adjusted group. Our experiment effectively enhanced soluble hRANKL expression in E. coli and might constitute a meaningful attempt to obtain soluble expression of recombinant protein in E. coli. |
| Starting Page | 849 |
| Ending Page | 862 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://journals.im.ac.cn/cjbcn/ch/reader/create_pdf.aspx?falg=1&file_no=gc17050849&quarter_id=5&year_id=2017 |
| PubMed reference number | 28876039v1 |
| Alternate Webpage(s) | https://doi.org/10.13345/j.cjb.160465 |
| DOI | 10.13345/j.cjb.160465 |
| Journal | Sheng wu gong cheng xue bao = Chinese journal of biotechnology |
| Volume Number | 33 |
| Issue Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Axis vertebra Bone Diseases Bone neoplasms Lysis Recombinant Proteins Recombinants |
| Content Type | Text |
| Resource Type | Article |
