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Producción y purificación parcial de enzimas hidrolíticas de Aspergillus ficuum en fermentación sólida sobre residuos agroindustriales
| Content Provider | Semantic Scholar |
|---|---|
| Author | Costa, Marcia Torres, Marcelo Magariños, Haroldo Reyes, Alejandro Monsalvo |
| Copyright Year | 2010 |
| Abstract | In this paper, describes the production of the enzymes phytase, cellulase, xylanase and protease by Aspergillus ficuum DSM 932 strain, in solid state fermentation (SSF) using canola cake and cranberry pomace as substrates. The enzyme activity was used in each case as an indirect measure of the enzymes production. Canola meal turned out to be a better substrate for phytase, cellulase and xylanase, while cranberry pomace was found to be a potential substrate for protease. Various ultrafiltration operations were carried out, decreasing the cut off membranes out in order to purify partially extracts of enzymes phytase, cellulase and xylanase, obtained from canola meal. Phytase was found to have a size >100 kDa, whereas cellulase and xylanase activity present in the retained 10, 30 and 50 kDa, suggesting that isozymes of both complexes have molecular weights ranging between 10 and 100 kDa. |
| Starting Page | 163 |
| Ending Page | 175 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Volume Number | 12 |
| Alternate Webpage(s) | http://www.scielo.org.co/pdf/biote/v12n2/v12n2a13.pdf |
| Alternate Webpage(s) | https://revistas.unal.edu.co/index.php/biotecnologia/article/download/18558/19954 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |