Nonsense-mediated mRNA decay mutation in Aspergillus nidulans.

Content Provider	Semantic Scholar
Author	Morozov, Igor Y. Negrete-Urtasun, Susana Tilburn, Joan Jansen, Christine A. Caddick, Mark X. Arst, Herbert N.
Copyright Year	2006
Abstract	An Aspergillus nidulans mutation, designated nmdA1, has been selected as a partial suppressor of a frameshift mutation and shown to truncate the homologue of the Saccharomyces cerevisiae nonsense-mediated mRNA decay (NMD) surveillance component Nmd2p/Upf2p. nmdA1 elevates steady-state levels of premature termination codon-containing transcripts, as demonstrated using mutations in genes encoding xanthine dehydrogenase (hxA), urate oxidase (uaZ), the transcription factor mediating regulation of gene expression by ambient pH (pacC), and a protease involved in pH signal transduction (palB). nmdA1 can also stabilize pre-mRNA (unspliced) and wild-type transcripts of certain genes. Certain premature termination codon-containing transcripts which escape NMD are relatively stable, a feature more in common with certain nonsense codon-containing mammalian transcripts than with those in S. cerevisiae. As in S. cerevisiae, 5' nonsense codons are more effective at triggering NMD than 3' nonsense codons. Unlike the mammalian situation but in common with S. cerevisiae and other lower eukaryotes, A. nidulans is apparently impervious to the position of premature termination codons with respect to the 3' exon-exon junction.
File Format	PDF HTM / HTML
DOI	10.1128/EC.00220-06
PubMed reference number	16963627
Journal	Medline
Volume Number	5
Issue Number	11
Alternate Webpage(s)	http://ec.asm.org/content/5/11/1838.full.pdf
Alternate Webpage(s)	https://doi.org/10.1128/EC.00220-06
Journal	Eukaryotic cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article