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Inhibition of collagen-induced platelet aggregation by normal plasma.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nossel, Hymie L. Wilner, George D. Drillings, Michael |
| Copyright Year | 1971 |
| Abstract | Normal plasma has been found to inhibit the platelet aggregation-inducing effect of collagen in a time consuming reaction independent of temperature. Collagen treated with serum and washed has reduced reactivity which can be restored to normal by treatment with 1.5 M sodium chloride. On the basis of this result, it is suggested that inhibition results from adsorption to collagen of a plasma component. The inhibitory plasma component is destroyed at 56 degrees C, is unstable below pH 7, and migrates with the alpha globulins on starch block electrophoresis at pH 8.6. On the basis of ultrafiltration and sucrose density gradient ultracentrifugation studies, a molecular weight in the range of 330,000 is suggested and there may be an additional component of considerably greater size. Partial purification can be achieved by ion exchange chromatography. The purified fraction was completely inactivated by incubation with trypsin. Partially purified fractions inhibit cationic platelet aggregators such as collagen, polylysine, and hexadimethrine but do not affect anionic aggregators such as succinylated collagen and sodium stearate. Normal plasma and serum inhibit succinylated collagen and stearate. Stearate is inhibited by crystalline albumin and Cohn fraction IV-4. It is suggested that plasma proteins may regulate platelet adhesion to collagen and other vessel wall materials. |
| Starting Page | 1 |
| Ending Page | 4 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://dm5migu4zj3pb.cloudfront.net/manuscripts/106000/106711/JCI71106711.pdf |
| PubMed reference number | 4107268v1 |
| Volume Number | 50 |
| Issue Number | 10 |
| Journal | The Journal of clinical investigation |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alpha-Globulins Blood Platelet Disorders Blood Platelets Centrifugation, Density Gradient Electrophoresis Globulins Hexadimethrine Bromide Ion Exchange Ion-Exchange Chromatography Procedure Iontophoresis Molecular Weight Platelet aggregation Polylysine SODIUM STEARATE Sodium Chloride Starch Stearates Sucrose Ultracentrifugation Ultrafiltration (procedure) Unstable Medical Device Problem |
| Content Type | Text |
| Resource Type | Article |
