Nicotine increases intracellular calcium in rat hippocampal neurons via voltage-gated calcium channels

Content Provider	Semantic Scholar
Author	Barrantes, Georgina E. Murphy, Coleen T. Westwick, John Wonnacott, Susan
Copyright Year	1995
Abstract	The effect of nicotinic receptor activation on intracellular calcium concentrations ([Ca2+]i) was quantitated in populations of cultured hippocampal neurons loaded with Fura-2. Nicotine (50 microM) and cytisine (50 microM) increased [Ca2+]i by 100%. This response was abolished in the presence of the nicotinic antagonist methyllycaconitine (MLA) whereas KCl-evoked increases in [Ca2+]i were insensitive to MLA. Glial cultures were unaffected by nicotine, although they did respond to glutamate with increased [Ca2+]i. In hippocampal neurons, responses to nicotinic agonists and KCl were dependent on the presence of extracellular Ca2+ and were similarly sensitive (85% inhibition) to CdCl2. These results are consistent with the presence of functional nicotinic receptors on hippocampal neurons. The receptors appear to elevate [Ca2+]i by promoting the influx of extracellular Ca2+ through voltage-gated calcium channels.
Starting Page	101
Ending Page	104
Page Count	4
File Format	PDF HTM / HTML
DOI	10.1016/0304-3940(95)11859-U
PubMed reference number	7501232
Journal	Medline
Volume Number	196
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/030439409511859U
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/030439409511859U?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/0304-3940%2895%2911859-U
Journal	Neuroscience Letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article