NDLI: Self-gelatinizable copolymer immobilized glucose biosensor based on prussian blue modified graphite electrode.

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Self-gelatinizable copolymer immobilized glucose biosensor based on prussian blue modified graphite electrode.

Content Provider	Semantic Scholar
Author	Deng, Qiliang Li, Baoli Dong, S.
Copyright Year	1998
Abstract	A novel poly(vinyl alcohol) grafting 4-vinylpyridine self-gelatinizable copolymer was adapted to immobilize glucose oxidase. The reduction of hydrogen peroxide (H2O2) was detected at a Prussian Blue (PB) modified graphite electrode. A stable and sensitive glucose amperometric biosensor is described. The copolymer is a good biocompatible polymer in which the glucose oxidase retains high activity. Moreover, the copolymer can adhere firmly to the inorganic PB membrane. The sensor showed an apparent Michaelis-Menten constant of 18 +/- 0.2 mM and a maximum current density of 1.14 microA cm-2 mM-1. The linear range is from 5 microM to 4.5 mM glucose and the detection limit is 0.5 microM glucose. The catalytic efficiency of PB for the reduction of H2O2 is higher than that for the oxidation of H2O2. Glucose concentrations in serum samples from healthy persons and diabetic patients were determined using the sensor. The results compared well with those provided by the hospital using a spectroscopy method.
File Format	PDF HTM / HTML
DOI	10.1039/A803309I
PubMed reference number	10209888
Journal	Medline
Volume Number	123
Issue Number	10
Alternate Webpage(s)	http://lib3.dss.go.th/fulltext/Journal/analyst/Analyst1998/no.10/1988v123n10p1995-1999.pdf
Alternate Webpage(s)	https://doi.org/10.1039/A803309I
Journal	The Analyst
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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