NDLI: Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals.

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Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals.

Content Provider	Semantic Scholar
Author	Antas, Paulo Renato Zuquim Cardoso, Fernando Luís Lopes Oliveira, Eliane B. Gomes, Patrícia K. C. Cunha, Kátia S. Sarno, Euzenir Nunes Sampaio, Elizabeth Pereira
Copyright Year	2004
Abstract	The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r(2) = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://tspace.library.utoronto.ca/bitstream/1807/1981/1/oc04009.pdf
Alternate Webpage(s)	http://www.scielo.br/pdf/mioc/v99n1/v99n1a09.pdf
PubMed reference number	15057347v1
Volume Number	99
Issue Number	1
Journal	Memorias do Instituto Oswaldo Cruz
Language	English
Access Restriction	Open
Subject Keyword	Cellular Immunity Cellular Phone ELECTROSURGICAL DEVICE Genus Mycobacterium In Vitro [Publication Type] Interferon Type II Latent Tuberculosis Leukemia, B-Cell Mycobacterium tuberculosis Patients Purified Protein Derivative of Tuberculin cytokine varicella-zoster immune globulin
Content Type	Text
Resource Type	Article

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