[Determination of cyanamide residue in grapes and cherries by liquid chromatography-tandem mass spectrometry coupled with precolumn derivatization].

Content Provider	Semantic Scholar
Author	Lan, F. Liu, Chuande
Copyright Year	2018
Abstract	A method was developed for the quantitative determination of cyanamide in grapes and cherries by liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with dansyl chloride (DNS) precolumn derivatization. First, the samples were homogenized, and then extracted with ethyl acetate under ultrasonication. The water was removed using anhydrous sodium sulfate, and the extract was concentrated and derivatized with dansyl chloride under alkaline condition. The separation was performed on a Shimadzu Shim-pack XR-ODS column (75 mm×2.0 mm, 1.6 μm) with the mobile phases of methanol and 2 mmol/L ammonium acetate aqueous solution (containing 0.05% (v/v) formic acid) in a gradient elution mode. The identification and quantification of cyanamide were carried out by MS/MS in positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) mode. The calibration curves showed good linearities in the range of 0.01-1.0 mg/L with the correlation coefficients not less than 0.9990. The average recoveries of cyanamide spiked at 0.01, 0.05 and 1.0 mg/kg in cherries and grapes were between 75% and 81%, and the relative standard deviations (RSDs) were between 6.5% and 9.8%. Both the limits of quantification (LOQs) of the analytes were 0.01 mg/kg. The method is simple, rapid, accurate and suitable for the confirmation and quantification of cyanamide in cherries and grapes.
Starting Page	78
Ending Page	82
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.chrom-china.com/fileup/PDF/sp1709004.pdf
PubMed reference number	29582616v1
Alternate Webpage(s)	https://doi.org/10.3724/SP.J.1123.2017.09004
DOI	10.3724/sp.j.1123.2017.09004
Journal	Se pu = Chinese journal of chromatography
Volume Number	36
Issue Number	1
Language	English
Access Restriction	Open
Subject Keyword	Cherry - dietary Chloride Ion Concentrate Dosage Form Cyanamide Extraction Greater Than or Equal To Less Than Liquid Chromatography Methanol Multiple Myeloma Percent Volume per Volume Quantitation REHMANNIA GLUTINOSA ROOT 5 g in 100 g ORAL POWDER [Chun-Shim Haedoksan] Recovery - action SODIUM SULFATE ANHYDROUS Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Volume fraction ammonium acetate analyte confirmation - ResponseLevel dansyl chloride ethyl acetate formic acid mg/kg
Content Type	Text
Resource Type	Article