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Isolation of Group B Streptococcus in groundwater in the North West Province , South Africa
| Content Provider | Semantic Scholar |
|---|---|
| Author | Moloantoa, Masego Ateba, Collins Njie |
| Copyright Year | 2013 |
| Abstract | The normal hosts of Streptococcus agalactiae are humans and animals and are highly predominant as normal flora in the female genital tract, male urethra, pharynx as well as in gastrointestinal tract. However, Streptococcus agalactiae has been associated with a number of complications in humans ranging septicaemia, pneumonia, meningitis to still birth. The aim of study was to isolate and characterize antibiotic resistant Streptococcus agalactiae from groundwater in selected areas within the Northwest Province. A total of 25 groundwater samples were randomly collected from selected areas around the Northwest Province. Brain Heart Infusion (BHI) agar was used to isolate Streptococcus species. Standard preliminary tests (Gram staining and catalase test) and confirmatory tests (ProlexTM Streptococcal Grouping Rapid Latex Agglutination test kit) were used to determine the identities of Streptococcus agalactiae. The antibiotic susceptibility profiles of the isolates were determined on all positively identified Streptococcus agalactiae. A large proportion (52.5% to 87.5%) of the isolates from Dibate, Ramosadi, Vryburg and Motlhabeng and only 35% of those from Taung were Gram positive cocci. All the isolates from the different sampling stations were catalase negative. Similarly, all the isolates from Taung, Vryburg, Motlhabeng, Stella and Setlagole were oxidase negative. Large proportions (50% to 90%) of the isolates tested were positively identified using the latex agglutination test. A large proportion (71 -100%) of isolates from Taung, Setlagole, Stella and Motlhabeng were resistant to ampicillin and penicillin. Moreover, all the isolates from Stella and Taung were resistant to vancomycin and tetracycline. Despite the fact that a large proportion (75%) of the isolates from Taung were resistant to chloramphenicol, only a small proportion (23% to 37.5%) of the isolates from Stella, Setlagole and Dibate were resistant to this antibiotic. However, none of the isolates from Stella and Setlagole were resistant to clarithromycin. A large proportion (84.6% to 100%) of the isolates from Dibate, Motlabeng and Ramosadi were resistant to erythromycin and clarithromycin. [Moloantoa M, Ateba CN. Isolation of Group B Streptococcus in groundwater in the North West Province, South Africa. Life Sci J 2013;10(3):444-449] (ISSN:1097-8135). http://www.lifesciencesite.com. 65 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.lifesciencesite.com/lsj/life1003/065_19451life1003_444_449.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
