Chromosome Conformation Capture (3C) in Budding Yeast.

Content Provider	Semantic Scholar
Author	Belton, Jon-Matthew Dekker, Job
Copyright Year	2015
Abstract	Chromosome conformation capture (3C) is a method for studying chromosomal organization that takes advantage of formaldehyde cross-linking to measure the spatial association of two pieces of chromatin. The 3C method begins with whole-cell formaldehyde fixation of chromatin. After cell lysis, solubilized chromatin is digested with a type II restriction endonuclease, and cross-linked DNA fragments are ligated together. Cross-links are reversed by degradation with proteinase K, and chimeric DNA molecules are purified by standard phenol:chloroform extraction. The resulting 3C library represents chromatin fragments that may be separated by large genomic distances or located on different chromosomes, but are close enough in three-dimensional space for cross-linking. Locus-specific oligonucleotide primers are used to detect interactions of interest in the 3C library using end-point polymerase chain reaction (PCR).
File Format	PDF HTM / HTML
PubMed reference number	26034304
Journal	Medline
Volume Number	2015
Issue Number	6
Alternate Webpage(s)	http://cshprotocols.cshlp.org/content/2015/6/pdb.prot085175.full.pdf
Alternate Webpage(s)	https://doi.org/10.1101/pdb.prot085175
Journal	Cold Spring Harbor protocols
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article