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Validación del protocolo de análisis de diversidad genética en Leoncillos (Cebuella pygmaea) mediante el uso de marcadores moleculares microsatélites
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tituaña, Garzón Estefanía, Marcela |
| Copyright Year | 2016 |
| Abstract | Cebuella pygmaea also known as Pigmy Marmoset is considered the smallest species of primates in Ecuador which is currently considered in the IUCN list as a vulnerable species. Genetic diversity studies result fundamental for the conservation and stability of the species to guarantee their care and protection. If non-invasive sampling techniques (feces, hair or urine) are used for the study, this becomes critic as DNA extracted from these sources may have quality problems (degradation, inhibitors and contaminants) that make subsequent molecular analysis difficult and results reliability diminishes. This is aggravated if specific DNA markers are not available for the amplification of DNA of the tested species. The objective of the present study was to determine the required number of PCR replications to ensure an acceptable level of reliability of the obtained results using Pigmy Marmoset feces samples as DNA sources and heterologous primers designed to amplify microsatellites regions from Callithrix jaccus. Thirty five feces samples of Pigmy Marmosets from “Yanacocha” Rescue Center in Puyo were analyzed. DNA was extracted and several PCR replications were performed using five pairs of heterologous primers, following the multiple tubes approach. It was determined that the main types of error for the analysis of non-invasive samples correspond to a wrong genotyping due to the presence of false alleles and non-detection of heterozygotes. From the five primers used in this study only two are within the recommended ranges recommended in the literature (CJ11 and CJ14). The remaining three should not be used in studies of genetic diversity for C. pygmaea due to the number of replications needed to obtain results (CJ1, CJ7 y CJ15). It is expected that the results of this study help define appropriate protocols to reliably determine the genetic diversity of C. pygmaea populations and thereby make the right decisions for their conservation and determine a factor of genotype correspondence to improve genetic diversity analysis using the set of primers used in this study. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorio.usfq.edu.ec/bitstream/23000/5190/1/124619.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
