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The clinical significance of Aurora-A/STK15/BTAK expression in human esophageal squamous cell carcinoma.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tanaka, Eiji Hashimoto, Yosuke Ito, Tetsuo Okumura, Tomoyuki Kan, Takatsugu Watanabe, Go Imamura, Masayuki Inazawa, Johji Shimada, Yutaka |
| Copyright Year | 2005 |
| Abstract | PURPOSE Aurora-A/STK15/BTAK (Aurora-A) encodes a Serine/Threonine kinase associated with chromosomal distribution, and its up-regulation induces chromosomal instability thereby leading to aneuploidy and cell transformation in several types of cancer. In this study, we investigated the role of Aurora-A in human esophageal squamous cell carcinoma (ESCC). EXPERIMENTAL DESIGN The expression levels of Aurora-A mRNA were compared in 33 ESCC tissues with that in corresponding normal esophageal epithelium by semiquantitative reverse transcription-PCR, and the distribution patterns and expression levels of Aurora-A protein were immunohistochemically investigated in the ESCC tumors of 142 patients. The results were then separately compared with the clinicopathologic findings of the patients, and the expression of Aurora-A was examined in nine ESCC cell lines and a normal esophageal epithelial cell line using Western blot analysis. RESULTS The up-regulation of Aurora-A mRNA was found in 30% (10 of 33) of the tumors by semiquantitative reverse transcription-PCR, and protein up-regulation was found in 53% (75 of 142) of the patients by immunohistochemistry. mRNA and protein up-regulation of Aurora-A were correlated with distant lymph node metastasis (P = 0.05 and P = 0.04, respectively), and patients with Aurora-A mRNA or protein up-regulation had a poorer prognosis (P = 0.003 and P = 0.0009, respectively). Furthermore, multivariate analysis revealed that up-regulation of the Aurora-A protein was an independent prognostic factor. In addition, Aurora-A expression in all ESCC cell lines was higher than that in a normal esophageal epithelial cell line. CONCLUSIONS The up-regulation of Aurora-A expression may reflect the malignant behavior of ESCC and may prove useful information as a prognostic factor for ESCC patients. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://clincancerres.aacrjournals.org/content/clincanres/11/5/1827.full.pdf |
| PubMed reference number | 15756006v1 |
| Volume Number | 11 |
| Issue Number | 5 |
| Journal | Clinical cancer research : an official journal of the American Association for Cancer Research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anatomic Node Aneuploidy Aurora Kinase A Body tissue Chromosomal Instability Distant Lymph Node Keratinized squamous cell of esophagus Neoplasms Patients Prognostic Factors Reverse Transcription Squamous Epithelial Cells Squamous cell carcinoma of esophagus Threonine Transcriptional Activation Up-Regulation (Physiology) aurora kinase C lymph node metastases lymph nodes |
| Content Type | Text |
| Resource Type | Article |
