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Purification and characterization of thermostable agarase from Bacillus sp. BI-3, a thermophilic bacterium isolated from hot spring.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Jiang Sha, Yujie Seswita-Zilda, Dewi Hu, Qiushi He, Peiqing |
| Copyright Year | 2014 |
| Abstract | An extracellular agarase was purified from Bacillus sp. BI-3, a thermophilic agar-degrading bacterium isolated from a hot spring in Indonesia. The purified agarase revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular mass of 58 kDa. The optimum pH and temperature of the agarase were 6.4 and 70°C, respectively. The activity of the agarase was stable at high temperatures, and more than 50% activity was retained at 80°C for 15 min. Furthermore, the enzyme was stable in the pH range of 5.8-8.0, and more than 60% of the residual activity was retained. Significant activation of the agarase was observed in the presence of K(+), Na(+), Ca(2+), Mg(2+), and Sr(2+); on the other hand, Ba(2+), Zn(2+), Cu(2+), Mn(2+), Co(2+), Fe(2+), and EDTA inhibited or inactivated the enzyme activity. The components of the hydrolytic product analyzed by thin-layer chromatography showed that the agarase mainly produced neoagarobiose. This study is the first to present evidence of agarolytic activity in aerobic thermophilic bacteria. |
| Starting Page | 19 |
| Ending Page | 25 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 24064915 |
| Journal | Medline |
| Volume Number | 24 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://www.jmb.or.kr/submission/Journal/024/JMB024-01-04_FDOC_1.pdf |
| Alternate Webpage(s) | https://doi.org/10.4014/jmb.1308.08055 |
| Journal | Journal of microbiology and biotechnology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
