NDLI: Functional domain analysis of glass, a zinc-finger-containing transcription factor in Drosophila.

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Functional domain analysis of glass, a zinc-finger-containing transcription factor in Drosophila.

Content Provider	Semantic Scholar
Author	Tjian, Robert
Copyright Year	1995
Abstract	The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/92/14/6557.full.pdf
PubMed reference number	7604032v1
Volume Number	92
Issue Number	14
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Carboxyl Group Cell Culture Techniques Deletion Mutation Drosophila melanogaster TRANSCRIPTION FACTOR Transcription Coactivator Transcription, Genetic Transcriptional Activation Zinc Fingers dna binding
Content Type	Text
Resource Type	Article

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