NDLI: Antibacterial and Antioxidant Activity of Hydrolysate from Goat Milk Protein Hydrolized by Crude Bromelain Extract ]

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Antibacterial and Antioxidant Activity of Hydrolysate from Goat Milk Protein Hydrolized by Crude Bromelain Extract ]

Content Provider	Semantic Scholar
Author	Kusumaningtyas, Eni Widiastuti, Raphaella Kusumaningrum, Harsi Dewantari Suhartono, Dan Maggy Thenawidjaja
Copyright Year	2016
Abstract	Goat milk is highly nutritious foodstuffs that beneficial for improving health. The milk contains b ioactive peptides which produced by hydrolysis process. The aim of this study was to evaluate antibacterial and antioxidant activities of hydrolisate produced from hydrolysis of goat milk protein by crude bromelain extract. Hydrolysis of goat milk protein was conducted using crude bromelain (0.1 U/mL) at pH 6, 50°C for 60 min. Hydrolysate was fractionated by using membrane molecular weight cut off 10 kD a. hydrolysate before and after fractionation were assayed for antibacterial and antioxidant activities. Toxicity of the Hydrolysate was determined by hemolysis assay. The result showed that the hydrolysate before and after fractionation inhib ited growth of E. coli, S. Typhimurium and L. monocytogenes. Hydrolysate after fractionation has higher antibacterial activity indicated that fractionation was able to improve antibacterial activities of the hydrolysate fraction. The hydrolysate showed scavenging activity to ABTS and DPPH radicals. Fraction <10 kDa has the highest antioxidant activity against both ABTS and DPPH radicals. Hemolysis assay showed that hydrolysate before and after fractionation did not cause lysis of red b lood cells, indicating safe for application. Both fraction <10 kDa and >10 kDa not only showed absence of hemolysis but also they were able to reduce autolysis of red b lood cells. The result showed that hydrolysate from goat milk hydrolyzed by bromelain were able to be antibacterial and antioxidant.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://journal.ipb.ac.id/index.php/jtip/article/download/11390/9145
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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