Sub-second oscillations of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate during platelet activation by ADP and thrombin: lack of correlation with calcium kinetics.

Content Provider	Semantic Scholar
Author	Raha, Sandip Jones, Gabriele D. Gear, Adrian R. L.
Copyright Year	1993
Abstract	The hypothesis that ADP and thrombin liberate Ins(1,4,5)P3 in blood platelets, with kinetics consistent for releasing Ca2+ within 2s, was tested by quenched-flow techniques. Both agonists stimulated transient and equal synthesis of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 near 200 ms and later short-lived peaks, which were not correlated with the slower steady increase in intracellular [Ca2+] between 0.5 to 2 s detected by Indo-1. Shear forces alone caused transient liberation of these inositol phosphates within 0.5 s and up to 4 s, yet failed to increase intracellular [Ca2+].
Starting Page	663
Ending Page	700
Page Count	38
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.biochemj.org/content/ppbiochemj/292/3/643.full.pdf
PubMed reference number	8317994v1
Volume Number	292
Part	3
Journal	The Biochemical journal
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Diphosphate Blood Platelets Calcium ion Indomethacin Inositol 1,4,5-Trisphosphate Inositol Phosphates Kinetics (discipline) Platelet Activation Supernumerary maxillary right second molar inorganic phosphate inositol-1,3,4,5-tetrakisphosphate
Content Type	Text
Resource Type	Article