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Formação in vitro da rede de fibrina durante ativação do plasma rico em plaquetas com cloreto de cálcio a 10% em temperatura de 37°C
| Content Provider | Semantic Scholar |
|---|---|
| Author | Leão, Moira Pedroso |
| Copyright Year | 2002 |
| Abstract | Research that measures coagulation time of plasma rich in platelets (FRF) obtained with the use of 10% calcium chloride through a coagulation measurement device. Its objective is to analyze the possibility of registering, through a coagulation measurement device, the formation of a FRF fibrin net; using only a sterile solution calcium chloride activation and verify if the constant 37 degrees Celsius temperature can provide the technique with a higher level of previewing the necessary time for coagulation. 8 samples of plasma rich in platelet were obtained through the collecting and centrifugation of healthy voluntary blood according to the Hospital de Clínicas of Curitiba Blood Bank standard protocol. One sample of 1 ml of plasma rich in platelets was pipetted from a test tube and taken to pre-heating at 37 degrees Celsius for 5 minutes. After the heating, the sample was mixed by inversion with 40-ml calcium chloride 10%, which had previously been pre-heated at 37 degrees Celsius. This mixture remained in a test tube for incubation at 37 degrees Celsius for 4 more minutes, when then again pipetted so that 200 |jl of this mixture were put in each one of the 4 cuvetes of a coagulation measurement device. This device has a system of dry heating at 37 degrees Celsius and, through an electromagnetic system, detects the beginning of the fibrin net formation by hindering the moving of the metal bilha put inside the cuvetes. In just one of the 8 healthy plasmas evaluated the time of incubation after the mixture was of only 2 minutes, once the coagulation of this sample was obtained with an average time of 130 seconds. The results revealed that the necessary time to promote the coagulation formation varied approximately from 2 to 10 minutes in patients studied after the mixture of plasma rich in platelets with 10% calcium chloride, when using heating at 37 degrees Celsius. It can be concluded that although the fibrin net formation compared to thrombin, this time can be administered by the surgeon without transmitting the to the technique the risks concerning allogenous or bovine origin material. New researches are necessary in order to evaluate the reactions occurring during the coagulation formation more precisely and if the pre-heating at 37 degrees Celsius alters in any way the desirable characteristics of growing factors, which are necessary to promote tissue repair. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/30363630.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
