Effets D'un Dextran Substitué Sur La Production De Feuillets Dermiques Et Du Gel De Fibrine Sur La Qualité Des Feuillets D'épidermes Cultivés

Content Provider	Semantic Scholar
Author	Boucher, Éric Le
Copyright Year	2005
Abstract	Extensive burns destroy the protective barrier of the skin, rendering the organism susceptible to bacterial infection. Important fluidic and loss of proteins can also ensue, leading to high metabolic stress. Thus, rapid restoration of this barrier is crucial to patient recovery. The preferred approach involves grafting autologous split-thickness skin over the wound bed. This approach is however greatly limited by the surface area of the body that was not affected by burns. One method used to circumvent this problem involves accelerating the regeneration of the donor sites by grafting them with cultured autologous epithelial sheets. Traditional epithelial sheets culture requires a dispase treatment that could potentially be detrimental to cell viability and requires costly logistics. Culturing epithelial cells on a readily graftable substrate, such as fibrin gel can side step this costly procedure. Epithelial sheets cultured on fibrin gels have already been successfully grafted onto patients in Europe. Moreover, in vitro testing revealed that sub-confluent epithelial cells cultured on a fibrin matrix share similar proliferative potential and differentiation markers with those cultured on plastic. This study compares fully confluent epithelial sheets cultured on plastic and on fibrin. We found significant histological differences between fibrin and plastic cultured epithelial sheets, likely due to contraction of the plastic cultured epithelial sheet once it was detached. Immunohistological analyses did not reveal any significant differences between the epithelial sheets produced by the two culture systems. Thus, our results show that epithelial cells cultured on a fibrin matrix can produce graftable, fully confluent epithelial sheets.
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Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article