Enzymatic preparation of human milk fat substitutes and their oxidation stability.

Content Provider	Semantic Scholar
Author	Kotani, Kousuke Yamamoto, Yukihiro Hara, Setsuko
Copyright Year	2015
Abstract	Human milk fat substitutes (HMFSs), rich in palmitic acid (P) at the sn-2 position of triacylglycerol (TAG), were prepared from lard via Novozym435(®)- and Lipozyme RM-IM(®)-mediated two-step reactions. First, 2-palmitoyl monoacylglycerol (2-P-MAG, 90% purity) was prepared via Novozym435(®)mediated ethanolysis of lard. Then, 2-P-MAG, oleic acid (O), linoleic acid (L), and lard were dissolved in hexane and subjected to a Lipozyme RM-IM(®)-mediated reaction for HMFS preparation. The effect of the amount of 2-P-MAG and fatty acids (O and L) in HMFS preparation were investigated. Under the optimum reaction conditions: 7 mmol of lard, 3.0 mmol of 2-P-MAG, 5.2 mmol of O, 3.5 mmol of L, 10 mL of hexane, 10 wt% of Lipozyme RM-IMR(®) (against the total weight of substrates), 550 rpm, 37°C and 6 h, a HMFS with total fatty acid composition and P content at the sn-2 position of TAG similar to that of human milk fat was prepared. In the same way, a HMFS having polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (PUFA-HMFS) was prepared. The HMFS and PUFA-HMFS prepared in this study, as well as fats extracted from commercially available powdered milk for infants (FPM) were evaluated for their oxidation stability by an auto-oxidation test. The test showed HMFS and PUFA-HMFS to possess greater oxidation stability than that the FPMs. These results indicated that the HMFS and PUFA-HMFS prepared in this study have value as potential ingredients for powdered milk.
Starting Page	16
Ending Page	27
Page Count	12
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/jos/64/3/64_ess14254/_pdf
PubMed reference number	25757431v1
Alternate Webpage(s)	https://doi.org/10.5650/jos.ess14254
DOI	10.5650/jos.ess14254
Journal	Journal of oleo science
Volume Number	64
Issue Number	3
Language	English
Access Restriction	Open
Subject Keyword	C10orf90 gene Eicosapentaenoic Acid Extraction Fat Substitutes Linoleic Acid Lipozyme MILK FAT Milk, Human Oleic Acid Palmitic Acid Polyunsaturated Fatty Acids Purity Ravens colored progressive matrices TNFRSF25 protein, human Thioctic Acid Triglycerides docosahexaenoic acid millimole monoacylglycerol biosynthetic process n-hexane oxidation
Content Type	Text
Resource Type	Article