NDLI: Genotyping of Toxoplasma gondii strain directly from human CSF samples of congenital toxoplasmosis clinical case.

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Genotyping of Toxoplasma gondii strain directly from human CSF samples of congenital toxoplasmosis clinical case.

Content Provider	Semantic Scholar
Author	Pagliuca, Chiara Pastore, Gabiria Scaglione, Elena Migliucci, Annalisa Maruotti, Giuseppe Maria Cicatiello, Annunziata Gaetana Salvatore, Elena Picardi, Marco Sammartino, Josè Camilla Buonocore, Maria Consiglio Martinelli, P. Iaccarino, Emilia Colicchio, Roberta Salvatore, Paola Di
Copyright Year	2017
Abstract	This report describes a case of congenital toxoplasmosis in a newborn in Southern Italy. A pregnant mother had been admitted at the 20th week of her pregnancy on account of pharyngodynia and laterocervical lymphadenopathy. Although serological testing of the mother's serum documented a seroconversion with positive IgG and IgM anti-Toxoplasma antibodies during II trimester, the woman refused to perform prenatal diagnosis for congenital toxoplasmosis. Fetal ultrasound scan already showed mild asymmetrical triventricular hydrocephaly and cerebral calcifications. After birth, real-time PCR on cerebrospinal fluid and blood samples of the newborn showed a positive result for 529bp-repeat element DNA of T. gondii, In addition brain magnetic resonance imaging and computed tomography showed a characteristic diffuse brain tissue loss associated with hydrocephalus. For the first time molecular characterization of T. gondii isolate was performed directly from the newborn's CSF samples by using nested-PCR-RFLP of sag-2 and pk1 genes. The PCR-RLFP analysis revealed that the isolate belongs to the clonal type II, the predominant lineage causing human toxoplasmosis, as confirmed by DNA sequencing.
Starting Page	151
Ending Page	154
Page Count	4
File Format	PDF HTM / HTML
PubMed reference number	28368078
Journal	Medline
Volume Number	40
Issue Number	2
Alternate Webpage(s)	http://www.newmicrobiologica.org/PUB/allegati_pdf/2017/2/151.pdf
Alternate Webpage(s)	http://www.newmicrobiologica.org/PUB/allegati_pdf/AOP/496M907.pdf
Journal	The new microbiologica
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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