NDLI: Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria / Gracilariopsis lemaneiformis ( Gracilariales , Rhodophyta )

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Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria / Gracilariopsis lemaneiformis ( Gracilariales , Rhodophyta )

Content Provider	Semantic Scholar
Author	Ren, Xueying Zhang, Xuecheng
Copyright Year	2006
Abstract	* Corresponding author Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macroarrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples, including 7 genes up-regulated in tetrasporophytes, 1 expressed exclusively in tetrasporophytes and 3 up-regulated in female gametophytes. By densitometric analysis relative to GAPDH, 8 cDNAs increased 1.3-4.2 fold and 3 decreased about 0.4-0.7 fold in tetrasporophytes compared to female gametophytes. The present study provides the first insight into genes that may involve in phase differentiation in G. lemaneiformis.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.bioline.org.br/pdf?ej06015
Alternate Webpage(s)	https://scielo.conicyt.cl/pdf/ejb/v9n2/a06.pdf
Alternate Webpage(s)	http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/viewFile/v9n2-11/247
Alternate Webpage(s)	http://ejb.ucv.cl/content/vol9/issue2/full/11/11.pdf
Language	English
Access Restriction	Open
Subject Keyword	Crossbreeding Database GAPDH gene Gracilaria Gracilariales Guanosine Triphosphate Phosphohydrolases Homology (biology) Hypothetical protein In-phase and quadrature components Metabolic Process, Cellular Nucleic Acid Hybridization Projection screen Rhodophyta Subtractive Hybridization Techniques Zero suppression
Content Type	Text
Resource Type	Article

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