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Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cereghino, Joan Lin Cregg, James Michael |
| Copyright Year | 2000 |
| Abstract | During the past 15 years, the methylotrophic yeast Pichia pastoris has developed into a highly successful system for the production of a variety of heterologous proteins. The increasing popularity of this particular expression system can be attributed to several factors, most importantly: (1) the simplicity of techniques needed for the molecular genetic manipulation of P. pastoris and their similarity to those of Saccharomyces cerevisiae, one of the most well-characterized experimental systems in modern biology; (2) the ability of P. pastoris to produce foreign proteins at high levels, either intracellularly or extracellularly; (3) the capability of performing many eukaryotic post-translational modifications, such as glycosylation, disulfide bond formation and proteolytic processing; and (4) the availability of the expression system as a commercially available kit. In this paper, we review the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced. We also describe new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system. |
| Starting Page | 45 |
| Ending Page | 66 |
| Page Count | 22 |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1574-6976.2000.tb00532.x |
| PubMed reference number | 10640598 |
| Journal | Medline |
| Volume Number | 24 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://biotechlab.ch/userfiles/file/787339_ref_cererghinocregg2000.pdf |
| Alternate Webpage(s) | https://watermark.silverchair.com/24-1-45.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAk4wggJKBgkqhkiG9w0BBwagggI7MIICNwIBADCCAjAGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6VWlgKKr7VVf0JbxAgEQgIICAfQvgG2vnfDaKw2bxZEOM4NbKnv2JF0Wa-PliOMoibwB_ILXDjkYrCsyHmE7LKdhiUguVUitqvH--4-2i3Kwp-tFe8XjK7FDvc9lZH34JZhvSHgCa_QHri_JBvQ7lZTo0wda8OgS2PWgSKH_9zi208v4BwE1AIZIksP1CyjjRfCP56n_FsSpq0W3TC-DEvPwbxlAtlDgIWJF0_xuJ7B5WinGxY7RfmI_W0iUianWvoXAZ_vtxIsIJbuYESLXFxbkc46vD5F6PCqPACuo6LOoSAC_vxUidi45sMTA7nTFT3ko1Su1A6u4h7dNHk_0VKUhy-GMUc3nhSeJxEax0Kh_CiSwmG8vwMoj7tu83Zbyzro7UcwF7Zsyof8rv3E50wsgs3GxK1VDmLEAiwLRWrZ57lXmmakzGLE9UHijgU7MIhNClm6ZAhCSCj4IpRiA1oZI5BuQcx0RphBsSaZrXov0LNBook8ecmoD_0EfHrICS9_M2Fc6FNRk6N7nW-WByZGV3s97D3fmgtN0WVJBIHEc_USXU9WqIoTldZ5KbtiQXpDbfmVV98V6YRvsQLvpvD5efqtfKjRWwUl5AuwV7saB560nLl5dXDHnLX0diQjsAH-tliIHWR6D2lh-PbKArVN1Mn7Az9kN5mFxi9nzPdpPhTCmQH-nZyl5IyId0LHqCQXi_g |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/femsre/24/1/10.1111/j.1574-6976.2000.tb00532.x/2/24-1-45.pdf?Expires=1493037910&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=PQzQGkLhD6CjASKjwec9GDwP6~qijpvYyDUxz9ejUmMe~0yPpGHn72c8QLXf7E8KCWYM-CONbNEA~PLKPZk5QVyDOR--qKvFENN4Qm9lcqIUyDeffUS6plDkdr4nIuv4wfLa78yJF6k4ufoFQjHtO84Dp6eU~vAZBjIfOr8XaSIL5vJo94j6Cl2Fb5qJDJiMsggPdupLdLj7EaXVSXOJn35L81efMqiZX0hc6pGNizd0VRCSzV0woVmwKW2C1RrykoMLchOkE7dGuR2SiRndVm4rsXa7FrpoFNSq6eLZvaRvUM6JdZmI5Y9niZeNsmI8PxYTDA3UhjQ91htNc2268Q__ |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1574-6976.2000.tb00532.x |
| Journal | FEMS microbiology reviews |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
