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Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Durieu, P. Ochatt, Sergio J. |
| Copyright Year | 2000 |
| Abstract | Large numbers of viable protoplasts of pea (Pisum sativum) and grass pea (Lathyrus sativus) were efficiently and reproducibly obtained and, for the first time, fused. Different procedures for fusion were compared, based either on electrofusion (750, 1000, 1250 or 1500 V cm(-1)), or on the use of macro or micromethods with a polyethylene glycol (PEG 6000 or PEG 1540), or a glycine/high pH solution. Over 10% of viable heterokaryons were obtained, with PEG as the most efficient and reproducible agent for protoplast fusion (>20% of viable heterokaryons). Both the division of heterokaryons and the formation of small calluses were observed. |
| Starting Page | 58 |
| Ending Page | 62 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jxb/51/348/10.1093/jexbot/51.348.1237/2/511237.pdf?Expires=1492499813&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=cubz2BLM5LE2XB-92Mu8LhjlCJLPYRb2mcJTtTT4kXbodeBVEE2UGPpakC3swyLMqJ2mhuukhadjIhkZxdtpZ-748WkIb9b4Pddk8vSp4tMb8hTd2mNGuIU4WvbANK62CXixWgHrxIdDEJDNdLl1tRLBKrzkFmGRq8QiCGCQVeRRlWAWG6MVPlmUJIuH86ICvK~hCU-zP8fSzDuL3i29j019kPj9ySBAQezp2eKYywl2~Jb47FSR8PVjyCGKNkUsdEwN7V74Rjl74-Sl2ozaQjfM37LgUHT-leFsZavcEosUtVz~Zs8SI67evFwlUhPVrfHJuLxTOx1ZnZX83SdFmQ__ |
| PubMed reference number | 10937699v1 |
| Volume Number | 51 |
| Issue Number | 348 |
| Journal | Journal of experimental botany |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Glycine Grass koehlers Ab.IgE:ACnc:Pt:Ser:Qn Lathyrus sativus One Thousand Peas - dietary Pisum sativum Polyethylene Glycols Protoplasts Skin callus |
| Content Type | Text |
| Resource Type | Article |