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Method of Making and Using Same Background of the Invention
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bell, Michael L. Chung, Roy |
| Copyright Year | 2017 |
| Abstract | Kearney et al., J. Immunology, vol. 114, No. 4, Apr. 1975, pp. 1143-1146. Kessler, J. Immunology, vol. 115, No. 6, Dec. 1975, pp. 1617-1624. Kessler, J. Immunology, vol. 117, No. 5, Part I, Nov. 1976, pp. 1482-1490. Primary Examiner-Christine M. Nucker Attorney, Agent, or Firm-Mahoney & Schick 57 ABSTRACT A stabilized radioimmunoassay product consisting of an antibody protein-bound to the cell wall of a selected bacterium whereby the antibody is irreversibly bound to the protein and remains specific for the antigen against which it was developed. The radioimmunoassay product is also characterized by the fact that the resul tant complete product includes a phase of serum protein treatment to isolate the protein sites or other sites of nonspecific reactivity with the labelled antigen such that the non-specific binding of labelled antigen is sig nificantly reduced. Also within the contemplation of the invention is the method of manufacturing the radio immunoassay product which includes the initial phase of protein binding of the antibody to the selected bacte rium and the subsequent serum treatment to isolate previously unutilized protein sites such that when said sites are subsequently exposed to labelled antigen it will be rejected. Further within the contemplation of the invention is the method of utilizing the aforesaid prod uct to totally eliminate the necessity for the customary incubation stages necessitated by prior art radioimmu noassay techniques. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://patentimages.storage.googleapis.com/e9/24/58/36342900611964/US4399229.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
