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Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST).
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lau, Ming Woei Dale, Bruce E. |
| Copyright Year | 2009 |
| Abstract | Current technology using corn stover (CS) as feedstock, Ammonia Fiber Expansion (AFEX) as the pretreatment technology, and Saccharomyces cerevisiae 424A(LNH-ST) as the ethanologenic strain in Separate Hydrolysis and Fermentation was able to achieve 191.5 g EtOH/kg untreated CS, at an ethanol concentration of 40.0 g/L (5.1 vol/vol%) without washing of pretreated biomass, detoxification, or nutrient supplementation. Enzymatic hydrolysis at high solids loading was identified as the primary bottleneck affecting overall ethanol yield and titer. Degradation compounds in AFEX-pretreated biomass were shown to increase metabolic yield and specific ethanol production while decreasing the cell biomass generation. Nutrients inherently present in CS and those resulting from biomass processing are sufficient to support microbial growth during fermentation. This platform offers the potential to improve the economics of cellulosic ethanol production by reducing the costs associated with raw materials, process water, and capital equipment. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.0812364106 |
| PubMed reference number | 19164763 |
| Journal | Medline |
| Volume Number | 106 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://www.pnas.org/content/106/5/1368.full.pdf |
| Alternate Webpage(s) | http://www.pnas.org/content/106/5/1368.full.pdf?with-ds=yes |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.0812364106 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
