Marcel Florkin CONCENTRATED SALT SOLUTIONS THE SOLUBILITY OF FIBRINOGEN IN CHEMISTRY OF THE PROTEINS : VII

Content Provider	Semantic Scholar
Author	Florkin, Marcel
Copyright Year	2003
Abstract	In 1859, Denis (8) observed the appearance of a gelatinous precipitate which he called plasmine in blood saturated with respect to sodium chloride.’ Plasmine was studied by Leon Fredericq (11) in 1877. He noted that upon heat coagulation, proteins separated at two different temperatures and, therefore, suggested that plasmine consisted of two proteins, one of them the postulated protein in the blood concerned with its coagulation and called fibrinogen by Virchow. In 1879 Hammarsten (14) precipitated one of these proteins from blood plasma by half saturating it with respect to sodium chloride. This method has ever since been the classical procedure in the preparation of fibrinogen. Fibrinogen has generally been considered insoluble in water, but soluble in dilute salt solutions and, therefore, to belong to that class of substances, the globulins, which were first described by Denis (8) in the same monograph in which he described plasmine. The contention of De Waele (9) that fibrinogen is largely soluble in water involves both the characterization of this protein and the concept of solubility, and these we shall consider in terms of the measurements that are reported. The early observations upon the temperature of heat coagula-
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Language	English
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