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Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Library Preparation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ramani, Suchitra Obermoeller, Dawn Toloue, Masoud M. |
| Copyright Year | 2011 |
| Abstract | The generation of high quality next generation sequencing data begins with libraries that have the desired insert size and proper adapter ligation. Artifacts during library preparation can result in PCR duplicates, uneven read coverage and poor adapter ligation efficiencies. We examined this by generating and analyzing DNA sequences from Escherichia coli genomes. Our improved protocol significantly reduces bias, increases ligation efficiency, improves indexing flexibility and increases high throughput functionality in DNA libraries prepared for sequencing. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.nature.com/app_notes/nmeth/2011/111110/pdf/an8082.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Base Sequence Biopolymer Sequencing Display resolution Genome Indexes Libraries Ligation Massively-Parallel Sequencing Morphologic artifacts Next-generation network Throughput |
| Content Type | Text |
| Resource Type | Article |
