NDLI: Analytical identification of additional impurities in urinary-derived gonadotrophins.

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Analytical identification of additional impurities in urinary-derived gonadotrophins.

Content Provider	Semantic Scholar
Author	Bassett, Robert Lispi, Monica Ceccarelli, David Grimaldi, Luigi Mancinelli, Michele Martelli, Franco Dorsselaer, Alain Van
Copyright Year	2009
Abstract	Advances in proteomic technology have enabled contaminant proteins to be identified from complex protein mixtures. The purity of two purified urinary gonadotrophin products, human menopausal gonadotrophin (u-HMG) and human FSH (u-hFSH), was compared with a preparation of recombinant human FSH (r-hFSH). After separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), western blot analysis showed that the recombinant preparation contained only FSH, whereas the urine-derived preparations exhibited several non-FSH or LH-related bands. These urinary components were further investigated by a proteomic approach using two-dimensional SDS-PAGE followed by mass spectrometric identification. The proteomic approach detected a total of 23 non-gonadotrophin-related proteins, at variable levels in different batches of the urine-derived preparations. Of these, 16 co-purified proteins have not been previously reported to be present in urine-derived gonadotrophins. These results indicate that the process used to purify urinary gonadotrophins may not remove all non-gonadotrophin proteins. By using a comprehensive proteomic approach, it has been shown that the recombinant FSH preparation has greater purity than either of the urine-derived preparations.
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.aduc.it/generale/files/file/allegati/20091209-Rbm.pdf
PubMed reference number	19778474v1
Volume Number	19
Issue Number	3
Journal	Reproductive biomedicine online
Language	English
Access Restriction	Open
Subject Keyword	Bands Contain (action) Follicle Stimulating Hormone, Human Gel Electrophoresis (lab technique) Gonadotropins Menopause Menotropins Polyacrylamide Gel Electrophoresis Purity Recombinants SDS-PAGE Sodium Dodecyl Sulfate Sulfate measurement Urofollitropin Western Blotting mixture polyacrylamide gels
Content Type	Text
Resource Type	Article

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