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Mitochondria of Isolated Plant Cells (Acer pseudoplatanus L.): II. Copper Deficiency Effects on Cytochrome C Oxidase and Oxygen Uptake.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bligny, Richard Douce, Roland |
| Copyright Year | 1977 |
| Abstract | The effects of copper deficiency on cell culture growth, cell respiration, mitochondrial oxidative properties, and electron transport chain have been studied with suspension-cultured sycamore cells (Acer pseudoplatanus L.). Within the range of the copper concentration studied (0.1-25 mug/1 of culture medium), the mean rate of cell division is independent of copper concentration. An initial copper concentration lower than 2 mug/1 limited the maximum density of population reached at the stationary phase of growth.On a protein basis, the uncoupled O(2) uptake rates were about the same for normal and copper-deficient cells. In contrast, the half-maximal inhibition of O(2) uptake rate was obtained at greater KCN concentration in the normal cells (20 muM) compared to copper-deficient cells (2 muM). Similar results were obtained with the normal and copper-deficient sycamore cell mitochondria.In the copper-deficient mitochondria, the concentration of the cytochrome aa(3) was less than 0.02 nmol/mg mitochondrial protein or 1/20 of the normal rate. The b- and c-type cytochrome content was invariant with copper depletion. It appeared that cytochrome aa(3) is present in large excess in normal cells. This work also indicated that cytochrome c is a very mobile molecule. |
| Starting Page | 10706 |
| Ending Page | 10711 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.plantphysiol.org/content/plantphysiol/60/5/675.full.pdf |
| Alternate Webpage(s) | http://www.plantphysiol.org/content/60/5/675.full.pdf |
| PubMed reference number | 16660162v1 |
| Volume Number | 60 |
| Issue Number | 5 |
| Journal | Plant physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acer Cell Culture Techniques Cell Respiration Cell division Culture Media Cytochrome-c Oxidase Deficiency Deficiency of glucose-6-phosphate dehydrogenase Electron Transport G1 to G0 transition Hypocupremia Millimole per Kilogram Oxygen Suspensions |
| Content Type | Text |
| Resource Type | Article |
