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Some aspects of the sequestration of cardenolides in the large milkweed bug, Oncopeltus fasciatus (Dallas) (Hemiptera: Lygaeidae)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Moore, Lynn V. |
| Copyright Year | 1985 |
| Abstract | S p e c i f i c a s p e c t s o f the s e l e c t i v e s e q u e s t r a t i o n , e x c r e t i o n and t o l e r a n c e o f c a r d e n o l i d e s i n the l a r g e mi lkweed b u g , O n c o p e l t u s f a s c i a t u s have been s t u d i e d u s i n g s p e c t r o p h o t o m e t r y a s s a y s , t h i n l a y e r c h r o m a t o g r a p h y , t r a c e r s t u d i e s , i n v i v o t o l e r a n c e a s s a y s , and enzyme i n h i b i t i o n t e c h n i q u e s . The c a r d e n o l i d e c o n t e n t o f t h e d o r s o l a t e r a l s p a c e , g u t , wings and f a t body o f O n c o p e l t u s f a s c i a t u s was e x a m i n e d . The r e s u l t s i n d i c a t e t h a t t h e m a j o r i t y o f c a r d e n o l i d e s s e q u e s t e r e d i n the i n s e c t a r e c o n c e n t r a t e d i n t h e d o r s o l a t e r a l s p a c e , w h i c h c o n f i r m s t h e b a s i c p a t t e r n o f q u a n t i t a t i v e d i s t r i b u t i o n o f c a r d e n o l i d e s i n fJ. f a s c i a t u s d e t e r m i n e d i n e a r l i e r w o r k . L a r g e amounts o f c a r d e n o l i d e s were n o t f o u n d i n t h e g u t , wings and f a t b o d y . The f emale f a t body c o n t a i n e d 4-5% o f the t o t a l c a r d e n o l i d e c o n t e n t o f the i n s e c t . The c a r d e n o l i d e c o n t e n t o f male f a t b o d y , and gut and wings o f bo th sexes was below t h e d e t e c t i o n l i m i t o f t h e c a r d e n o l i d e a s s a y . T h i n l a y e r c h r o m a t o g r a p h y was used t o d e t e r m i n e t h e c a r d e n o l i d e a r r a y o f v a r i o u s t i s s u e s and s e c r e t i o n s o f fJ. f a s c i a t u s r e a r e d on seeds o f a s i n g l e s p e c i e s o f mi lkweed ( A . s p e c i o s a ) and a d u l t e x t r a c t s and d o r s o l a t e r a l space f l u i d o f 0. f a s c i a t u s r e a r e d on seeds o f two s p e c i e s o f mi lkweed w i t h d i f f e r e n t c a r d e n o l i d e a r r a y s ( A . s p e c i o s a and A . s y r i a c a ) • The r e s u l t s i n d i c a t e t h a t c a r d e n o l i d e s a r e n o t s e q u e s t e r e d i n the i n s e c t s i m p l y on t h e b a s i s o f p o l a r i t y and t h a t m e t a b o l i s m and d i f f e r e n t i a l e x c r e t i o n o f c a r d e n o l i d e s a r e i n v o l v e d i n t h e s e q u e s t r a t i o n o f c a r d e n o l i d e s i n fJ. f a s c i a t u s . . The s i m i l a r i t i e s i n t h e c a r d e n o l i d e p r o f i l e s o f 0. f a s c i a t u s r e a r e d on d i f f e r e n t f o o d s o u r c e s , and t i s s u e s o f fj. f a s c i a t u s r e a r e d on a s i n g l e f o o d s o u r c e i n d i c a t e s t h a t t h e r e i s r e g u l a t i o n o f the c a r d e n o l i d e a r r a y i n 0. f a s c i a t u s . An i n v i t r o p r e p a r a t i o n o f M a l p i g h i a n |
| File Format | PDF HTM / HTML |
| DOI | 10.14288/1.0096733 |
| Alternate Webpage(s) | https://open.library.ubc.ca/media/download/pdf/831/1.0096733/1 |
| Alternate Webpage(s) | https://doi.org/10.14288/1.0096733 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |