Loading...
Please wait, while we are loading the content...
Similar Documents
Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wölk, Benno Gremion, Christel Ivashkina, Natalia Engler, Olivier B. Grabscheid, Benno Bieck, Elke Blum, H. E. Cerny, Andreas Moradpour, Darius |
| Copyright Year | 2005 |
| Abstract | The cellular immune response plays a central role in virus clearance and pathogenesis of liver disease in hepatitis C. The study of hepatitis C virus (HCV)-specific immune responses is limited by currently available cell-culture systems. Here, the establishment and characterization of stable human HLA-A2-positive B-lymphoblastoid x T hybrid cell lines constitutively expressing either the NS3-4A complex or the entire HCV polyprotein are reported. These cell lines, termed T1/NS3-4A and T1/HCVcon, respectively, were maintained in continuous culture for more than 1 year with stable characteristics. HCV structural and non-structural proteins were processed accurately, indicating that the cellular and viral proteolytic machineries are functional in these cell lines. Viral proteins were found in the cytoplasm in dot-like structures when expressed in the context of the HCV polyprotein or in a perinuclear fringe when the NS3-4A complex was expressed alone. T1/NS3-4A and T1/HCVcon cells were lysed efficiently by HCV-specific cytotoxic T lymphocytes from patients with hepatitis C and from human HLA-A2.1 transgenic mice immunized with a liposomal HCV vaccine, indicating that viral proteins are processed endogenously and presented efficiently via the major histocompatibility complex class I pathway. In conclusion, these cell lines represent a unique tool to study the cellular immune response, as well as to evaluate novel vaccine and immunotherapeutic strategies against HCV. |
| Starting Page | 137 |
| Ending Page | 137 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://vir.sgmjournals.org/content/86/6/1737.full.pdf |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/86/6/1737.pdf?accname=guest&checksum=1D33F23BD6F0B69869E7C747A068658B&expires=1543238374&id=id |
| PubMed reference number | 15914852v1 |
| Volume Number | 86 |
| Part | 6 |
| Journal | The Journal of general virology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell hybridization Cultured Cell Line HLA Antigens HLA-DQ Antigens Helper-Inducer T-Lymphocyte Hepatitis C virus Liposomes Liver diseases Lysis Patients Viral Proteins autologous Epstein-Barr virus-specific cytotoxic T lymphocytes (cell) varicella-zoster immune globulin |
| Content Type | Text |
| Resource Type | Article |
