NDLI: Intermolecular crosslinking of fatty acyl chains in phospholipids: use of photoactivable carbene precursors.

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Intermolecular crosslinking of fatty acyl chains in phospholipids: use of photoactivable carbene precursors.

Content Provider	Semantic Scholar
Author	Gupta, Chhitar Mal Radhakrishnan, Ramachandran Gerber, Gerhard E. Olsen, William L. Quay, Steven C. Khorana, Har Gobind
Copyright Year	1979
Abstract	Phospholipids containing photolysable carbene precursors (beta-trifluoro-alpha-diazopropionoxy and m-diazirinophenoxy groups) in omega-positions of sn-2 fatty acyl chains were prepared. Photolysis of their vesicles produced crosslinked products in 40-60% yields. Crosslinking was mostly intermolecular and occurred by carbene insertion into the C-H bonds of a second fatty acyl chain. Crosslinking products were characterized by (i) their gel permeation behavior, (ii) analysis of products formed by base-catalyzed transesterification, (iii) degradation with phospholipases A2 and C, (iv) gas chromatography/mass spectrometry, and (v) use of mixtures of phospholipids carrying the carbene precursors and a phospholipid containing radioactively labeled fatty acyl groups. Nitrenes generated from the aliphatic or aromatic azido groups in phospholipids were unsatisfactory for forming crosslinks by insertion in C-H bonds.
Starting Page	215
Ending Page	216
Page Count	2
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://repository.ias.ac.in/15956/1/341.pdf
PubMed reference number	288050v1
Volume Number	76
Issue Number	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	AROMATICS Clinical act of insertion Cross link Fatty Liver Flatulence Gas Chromatography-Mass Spectrometry Insertion Mutation Phospholipase Phospholipids Tracer Vesicle (morphologic abnormality) acyl-CoA dehydrogenase carbene photolysis type I interferon receptor
Content Type	Text
Resource Type	Article

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