NDLI: Visualization of the cbbL Gene in Healthy and Starved Chemoautotrophic Nitrifying Bacteria

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Visualization of the cbbL Gene in Healthy and Starved Chemoautotrophic Nitrifying Bacteria

Content Provider	Semantic Scholar
Author	Sinigalliano, Christopher D. Jones, R. D. Stevens Kuhn, David N. Guerrero, Maria Angelica Acevedo
Copyright Year	2003
Abstract	Three in situ methods of visualizing the cbbL gene in intact cells of nitrifying bacteria at different physiological states (dormant and metabolically active) were compared after epifluorescence microscopy and image analysis. FISH alone showed the weakest signal intensity. Direct in situ PCR, incorporating labeled nucleotides, showed the greatest sensitivity but also the greatest background. The combination of unlabeled in situ PCR followed by FISH showed relatively high sensitivity, along with the lowest background and highest specificity. Although functional gene expression was not examined in this study, visualization of the potential for carbon fixation in heterogeneous cultures of nitrifying bacteria was demonstrated. RID=”” ID=”” Correspondence to: C.D. Sinigalliano; email: sinigall@fiu.edu
Starting Page	77
Ending Page	83
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1007/s00284-002-3941-0
PubMed reference number	14506851
Journal	Medline
Volume Number	47
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/s00284-002-3941-0
Alternate Webpage(s)	https://doi.org/10.1007/s00284-002-3941-0
Journal	Current Microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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