NDLI: Short communication. An effcient axillary shoot induction system for the living fossil plant – Wollemi pine (Wollemia nobilis)

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Short communication. An effcient axillary shoot induction system for the living fossil plant – Wollemi pine (Wollemia nobilis)

Content Provider	Semantic Scholar
Author	Du, Jing-Chuan Zhang, Q. Pei, Xuanyuan Zhan, Xinhua
Copyright Year	2013
Abstract	Aim of study: The aim of the work was to determine the optimum axillary shoot induction system by optimizing different factors. Area of study: The different factors which were pretreatment methods, media types, kinds of cytokinins and dark treatment Material and methods: After pretreating and sterilizing, the stems of Wollemi pine which about 3 cm long were cultured on three kinds of media (GD, DCR and MS) with different concentration and type of cytokines (BA, ZT and TDZ), as well as dark treatment (0, and 4 weeks) and then exposure to tissue culture room. The experiments were randomized block designed and the results were analyzed with ANOVA. Main results: Rapid freezing in liquid nitrogen was the most effective pretreatment method, dark treatment for the first 4-weeks of stem explants cultured in induction media could increase the frequency of axillary shoot induction significantly. The highest frequency of axillary shoot induction (63%) was obtained when explants were cultured in 1/2GD medium with 6.8 μM TDZ combining 4-weeks dark treatment. Research highlights: The optimized conditions for shoot production in vitro can be useful for conservation in vitro of Wollemia nobilis. Key word: Wollemia nobilis; in vitro conservation; tissue culture; an endangered relict conifer. * Corresponding author: ny10601@126.com Received: 22-01-13. Accepted: 24-04-13. Abbreviations: GD-Gresshoff and Doy; DCR-Gupta and Durzan; MS-Murashige and Skoog; BA-6-Benzy ladenine; ZTZeatin; TDZ-Thidiazuron. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Forest Systems 2013 22(3): 564-567 Available online at www.inia.es/forestsystems ISSN: 2171-5068 http://dx.doi.org/10.5424/fs/2013223-03997 eISSN: 2171-9845
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://revistas.inia.es/index.php/fs/article/download/3997/1956
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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