NDLI: Quantitative estimation of interaction between carbohydrates and concanavalin A by surface plasmon resonance biosensor.

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Quantitative estimation of interaction between carbohydrates and concanavalin A by surface plasmon resonance biosensor.

Content Provider	Semantic Scholar
Author	Goto, Satoru Masuda, Kyoko Miura, Miyuki Kanazawa, Keiko Sasaki, Miki Masui, Miyuki Shiramizu, Maki Terada, Hiroshi Chuman, Hiroshi
Copyright Year	2002
Abstract	We measured the affinity of more than 20 sugars with concanavalin A (ConA) by an optical biosensor (surface plasmon resonance sensor) using asialofetuin (ASF) as an immobilized binding partner of ConA. We determined kinetic parameters of the effects of sugars on the dissociation of ConA from ASF quantitatively, and the structural requirements of the functional groups of sugars for binding with ConA. We found that the affinity of ConA for sugars is dependent on its conformation induced by interaction with the binding partner. In addition, the results showed that optical biosensor system is well mimics the interaction of ConA with sugars in biomembrane.
Starting Page	8217
Ending Page	8223
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cpb.pharm.or.jp/cpb/200204/c04_0445.pdf
PubMed reference number	11963988v1
Volume Number	50
Issue Number	4
Journal	Chemical & pharmaceutical bulletin
Language	English
Access Restriction	Open
Subject Keyword	Affinity Carbohydrate nutrients Concanavalin A Greater Than Kinetics Sugars Surface Plasmon Resonance asialofetuin
Content Type	Text
Resource Type	Article

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