Role of acyl-CoAs and acyl-CoA-binding protein in regulation of carbon supply for fatty acid biosynthesis.

Content Provider	Semantic Scholar
Author	Fox, Simon Richard Rawsthorne, Stephen Hills, Matthew J.
Copyright Year	2000
Abstract	Acyl-CoA esters inhibit the plastidial glucose 6-phosphate (Glc-6-P) transporter and the adenylate transporter; the IC(50) values for the inhibition by oleoyl-CoA (18:1-CoA) are 200-400 nM and 1-2 microM respectively. The inhibition of either of these processes significantly reduces the flux of carbon from Glc-6-P or from acetate into long-chain fatty acids. The effect is dependent on the acyl chain length, e.g. lauryl-CoA is less inhibitory than oleoyl-CoA, causing 34 and 68% inhibition respectively of Glc-6-P uptake after 30 s. The inhibition of Glc-6-P and ATP transport is alleviated by addition of an equivalent concentration of acyl-CoA-binding protein (ACBP) or BSA. Acyl-CoAs do not inhibit pyruvate or glucose transporters. The endogenous concentrations of acyl-CoAs and ACBP are similar during embryo maturation.
File Format	PDF HTM / HTML
DOI	10.1042/bst0280672
Alternate Webpage(s)	http://www.biochemsoctrans.org/content/ppbiost/28/6/672.full.pdf
PubMed reference number	11171165
Alternate Webpage(s)	https://doi.org/10.1042/bst0280672
Journal	Medline
Volume Number	28
Issue Number	6
Journal	Biochemical Society transactions
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article