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Electrophoretic force on a protein-coated DNA molecule in a solid-state nanopore.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hall, Adam R. Dorp, Stijn Van Lemay, Serge G. Dekker, Cees |
| Copyright Year | 2009 |
| Abstract | Using solid-state nanopores with optical tweezers, we perform force spectroscopy on DNA molecules that are coated with RecA proteins. We observe that the electrophoretic force is 2-4 times larger for RecA-DNA filaments than for uncoated DNA molecules and that this force increases at lower salt concentrations. The data demonstrate the efficacy of solid-state nanopores for locally probing the forces on DNA-bound proteins. Our results are described quantitatively by a model that treats the electrophoretic and hydrodynamic forces. The conductance steps that occur when RecA-DNA enters the nanopore change from conductance decreases at high salt to conductance increases at low salt, which allows the apparent charge of the RecA-DNA filament to be extracted. The combination of conductance measurements with local force spectroscopy increases the potential for future solid-state nanopore screening devices. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/nl9027318 |
| PubMed reference number | 19780587 |
| Journal | Medline |
| Volume Number | 9 |
| Issue Number | 12 |
| Alternate Webpage(s) | http://ceesdekkerlab.nl/wp-content/uploads/Hall_2009.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/nl9027318 |
| Journal | Nano letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
