NDLI: Salmonella enterica serovar typhimurium surA mutants are attenuated and effective live oral vaccines.

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Salmonella enterica serovar typhimurium surA mutants are attenuated and effective live oral vaccines.

Content Provider	Semantic Scholar
Author	Sydenham, Mark A. Douce, Gillian Bowe, Frances Ahmed, S. Chatfield, Steve Dougan, Gordon
Copyright Year	2000
Abstract	A previously described attenuated TnphoA mutant (BRD441) of Salmonella enterica serovar Typhimurium C5 (I. Miller, D. Maskell, C. Hormaeche, K. Johnson, D. Pickard, and G. Dougan, Infect. Immun. 57:2758-2763, 1989) was characterized, and the transposon was shown to be inserted in surA, a gene which encodes a peptidylprolyl-cis, trans-isomerase. A defined surA deletion mutation was introduced into S. enterica serovar Typhimurium C5 and the mutant strain, named S. enterica serovar Typhimurium BRD1115, was extensively characterized both in vitro and in vivo. S. enterica serovar Typhimurium BRD1115 was found to be defective in the ability to adhere to and invade eukaryotic cells. Furthermore, S. enterica serovar Typhimurium BRD1115 was attenuated by at least 3 log units when administered orally or intravenously to BALB/c mice. Complementation of the mutation with a plasmid carrying the intact surA gene almost completely restored the virulence of BRD1115. In addition, S. enterica serovar Typhimurium BRD1115 demonstrated potential as a vaccine candidate, since mice immunized with BRD1115 were protected against subsequent challenge with S. enterica serovar Typhimurium C5. S. enterica serovar Typhimurium BRD1115 also showed potential as a vehicle for the effective delivery of heterologous antigens, such as the nontoxic, protective fragment C domain of tetanus toxin, to the murine immune system.
File Format	PDF HTM / HTML
DOI	10.1128/IAI.68.3.1109-1115.2000
Alternate Webpage(s)	http://iai.asm.org/content/68/3/1109.full.pdf
PubMed reference number	10678914
Alternate Webpage(s)	https://doi.org/10.1128/IAI.68.3.1109-1115.2000
Journal	Medline
Volume Number	68
Issue Number	3
Journal	Infection and immunity
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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