Simulated microgravity alters rat mesenteric artery vasoconstrictor dynamics through an intracellular Ca(2+) release mechanism.

Content Provider	Semantic Scholar
Author	Colleran, Patrick N. Behnke, Bradley J. Wilkerson, Michael K. Donato, Anthony J. Delp, Michael
Copyright Year	2008
Abstract	Previous work has shown that orthostatic hypotension associated with cardiovascular deconditioning results from inadequate peripheral vasoconstriction. We used the hindlimb-unloaded (HU) rat in this study as a model to induce cardiovascular deconditioning. The purpose of this study was to test the hypothesis that 14 days of HU diminishes vasoconstrictor responsiveness of mesenteric resistance arteries. Mesenteric resistance arteries from control (n = 43) and HU (n = 44) rats were isolated, cannulated, and pressurized to 108 cm H(2)O for in vitro experimentation. Myogenic (intralumenal pressure ranging from 30 to 180 cm H(2)O), KCl (2-100 mM), norepinephrine (NE, 10(-9)-10(-4) M) and caffeine (1-20 mM) induced vasoconstriction, as well as the temporal dynamics of vasoconstriction to NE, were determined. The active myogenic and passive pressure responses were unaltered by HU when pressures remained within physiological range. However, vasoconstrictor responses to KCl, NE, and caffeine were diminished by HU, as well as the rate of constriction to NE (C, 14.8 +/- 3.6 microm/s vs. HU 7.6 +/- 1.8 microm/s). Expression of sarcoplasmic reticulum Ca(2+)ATPase 2 and ryanodine 3 receptor mRNA was unaffected by HU, while ryanodine 2 receptor mRNA and protein expression were diminished in mesenteric arteries from HU rats. These data suggest that HU-induced and microgravity-associated orthostatic intolerance may be due, in part, to an attenuated vasoconstrictor responsiveness of mesenteric resistance arteries resulting from a diminished ryanodine 2 receptor Ca(2+) release mechanism.
Starting Page	72
Ending Page	79
Page Count	8
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Alternate Webpage(s)	http://ajpregu.physiology.org/content/ajpregu/294/5/r1577.full.pdf
Alternate Webpage(s)	http://ajpregu.physiology.org/content/ajpregu/early/2008/03/19/ajpregu.00084.2008.full.pdf
Alternate Webpage(s)	http://ajpregu.physiology.org/content/ajpregu/294/5/R1577.full.pdf
PubMed reference number	18353882v1
Alternate Webpage(s)	https://doi.org/10.1152/ajpregu.00084.2008
DOI	10.1152/ajpregu.00084.2008
Journal	American journal of physiology. Regulatory, integrative and comparative physiology
Volume Number	294
Issue Number	5
Language	English
Access Restriction	Open
Subject Keyword	Arterial system Caffeine Calcium Cardiovascular Diseases In Vitro [Publication Type] Lactose Intolerance Lower Extremity Mesenteric Arteries Mesentery Norepinephrine Potassium Chloride Ryanodine Shy-Drager Syndrome Vascular constriction (function) Vasoconstrictor Agents protein expression
Content Type	Text
Resource Type	Article