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Production and characterization of a bacteriocin produced by Enterococcus faecium LR/6
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kumar, M. S. Vasanth Ghosh, Nitika Srivastava, Sheela |
| Copyright Year | 2009 |
| Abstract | A soil isolate of Lactic acid bacteria (LAB), strain LR/6 was identified as Enterococcus faecium on the basis of its morphological, biochemical, and 16S rDNA analysis. Strain LR/6 is a potential producer of an antimicrobial compound that followed the kinetic pattern typical of a primary metabolite synthesis and is released extracellularly. The antimicrobial action remained unaffected when treated with β-glycerophosphate and catalase suggesting that the activity is neither based on acid nor H2O2 production. This antimicrobial compound was highly stable over a wide range of pH (2.0 to 8.0), high temperature (boiling and autoclaving) and could be stored stably at a range of temperature (-20oC to 37oC) at least upto one year. The proteinaceous nature of the antimicrobial compound was ascertained by its sensitivity to many proteolytic enzymes confirming it to be a bacteriocin. Strain LR/6 bacteriocin was also insensitive to the presence of α-amylase, lipase, surfactants, organic solvents, and showed a bactericidal mode of action. Crude bacteriocin exhibited broad inhibition spectrum both against related as well as some foodborne pathogenic bacteria including Listeria monocytogenes. Tricine SDS-PAGE analysis and bacteriocin activity assay corresponded to a protein of an apparent molecular mass of ~6.0 kDa. |
| File Format | PDF HTM / HTML |
| DOI | 10.5580/1530 |
| Volume Number | 8 |
| Alternate Webpage(s) | https://print.ispub.com/api/0/ispub-article/8450 |
| Alternate Webpage(s) | https://doi.org/10.5580/1530 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
